S. Lokesh Rao Magar¹, G.Vandana², Sobha Devi³ , H.Sandhya Rani⁴, Sandhya Anil⁵

¹Associate professor, ^2,3Assistant Professor,⁴Professor & Head,5Professor, Department of Pathology,Kakatiya Medical College, Warangal, Telangana,India.

Address for correspondence: Dr. S.Lokesh Rao Magar, Assistant Professor, Department of Pathology,Kakatiya Medical College, Warangal, Telangana,India.

Email: magar.lokesh@yahoo.in

Background: To explore the expression of p16INK4a(p16) & survivin in cervical intraepithelial neoplasm (CIN) & cervical squamous neoplasm

Material and Methods : A 3years study was undertaken in MGM Hospital, Warangal, all the cervical & hysterectomy specimens were studied. By considering both exclusion & inclusion criteria 46 cases were taken for p16INK4A & survivin expression & data was analysed

Results :The p16INK4A & survivin expressions showed the presence of statistical significance in cervical cancer, CIN1, CINII, CINIII and a normal cervical tissue & the comparison also revealed statistical significance among group. P16INK4A showed increase intensity with grade ( CINI = 28.57%, CINII = 44.44%, CINIII = 70%, & SCC = 90%), whereas survivin expressions were positive for both nuclear as well as cytoplasm staining( CINI = 28.57%, CINII = 22.22%,CINIII = 40%, SCC = 75% ) . Moreover, p16INK4A protein was associated with CIN grade & in cervical cancer; survivin protein was also related with clinical stages, CIN grade; the p16INK4A & survivin expressions were positively correlated with cervical cancer, & associated with poor prognosis of cervical cancer.

Conclusion :Briefly, p16INK4A & survivin expressions were positively correlated with the clinic-pathological & prognosis of cervical cancer.P16INK4A linearly correlates well with increasing grade of CIN & SCC, whereas as survivin does not shows linear correlation with increasing grades of CIN . But it was the useful marker for differentiating CIN & SCC, especially in diagnostically difficult cases.

Keywords :P16INK4A, Survivin ,Carcinoma in-situ , Squamous cell carcinoma, cervix