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Original Articles

Study of p16ink4a expression in cervical intraepithelial lesions and cervical carcinoma

Year : 2018 | Volume : 6 | Issue : 2 Page : 51 - 56

L.Krishna1,B.Sridhar Reddy2,J.Ranjani3,B.H.P.C.Sekhar4,P.Madhusudana5

1Professor and H.O.D(Retd..),2Associate Professor,3,4Assistant Professor, Department of Pathology, 5Scientist,MDRU, Sri Venkateswara Medical College, Tirupathi, Chittoor District, Andhra Pradesh, India.

Address for correspondence: Dr. L. Krishna Professor and H.O.D.(Retired) S.V.Medical College. Tirupathi. 5-12-121/1, Mangapuram,H B Colony, Moulali, Hyderabad, T.S.



Bakground: Cervical cancer ranks as the second cause of female cancer in India. In India about 122,844 new cervical cancer cases are diagnosed annually. Many lines of evidence have demonstrated that HPV contributes to neoplastic progression through the action of two viral oncoproteins, namely E6 and E7, which interact with various cell cycle regulatory proteins. The p16Ink4a is a tumour suppressor protein and it’s over expression highlights the possible potential of a marker for cervical intraepithelial lesions and cervical cancer.

Objectives : The present study was done to establish the ability of P16INK4A as a marker of dysplastic and neoplastic alteration in the cervical epithelium.

Materials and Methods :Surgical biopsy specimens of uterine cervix received at the Department of Pathology S.V. Medical College Tirupati from November 2014 to October 2016 were analyzed in a Prospective interventional study. 163 cases diagnosed as LSIL, HSIL, SCC, Adenocarcinoma on H&E stained sections were immunohistochemically stained for p16Ink4a using the BiogenexLifesystems Histology Kit. All sections that showed either strong nuclear and/or cytoplasmic staining were considered positive and graded qualitatively. Also 20 random normal cervical biopsies were immunohistochemically stained for p16Ink4a and studied to confirm the negative association.

Results :Low Grade Squamous Intraepithelial (LSIL) is more associated with IHC grade 1, whereas High Grade Squamous Intraepithelial (HSIL) and Squamous cell carcinoma (SCC) were more associated with IHC grade 3.

Conclusion : The present study concludes that p16Ink4a is a specific marker for dysplastic and neoplastic alteration in the cervical epithelium and thereby significantly improves cervical precancer and cancer detection.

Keywords :Cervix Cancer, E7-Oncoprotein, HPV, IHC-p16Ink4a.


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