Volume: 13 Issue: 1
Year: 2025, Page: 29-34, Doi: https://doi.org/10.47799/pimr.1301.07
Received: June 26, 2024 Accepted: April 2, 2025 Published: April 10, 2025
Background: Aspergillosis is increasingly recognized as a serious opportunistic infection, particularly among immunocompromised patients. Accurate species-level identification of Aspergillus is critical for selecting appropriate antifungal therapy and implementing effective infection control measures. This study aimed to identify and characterize Aspergillus species isolated from both clinical specimens and indoor air samples at a tertiary care hospital in Hyderabad, Telangana, India. Methods: Over a one-year period, a total of 709 specimens—including nasal mucosa swabs, tissue biopsies (lung, FESS), blood samples, nail clippings, and passive indoor air settle plates—were processed in the microbiology laboratory. Direct microscopic examination was performed using KOH mount, periodic acid–Schiff stain, and calcofluor white stain. Specimens showing filamentous fungi were cultured on Sabouraud dextrose agar, potato dextrose agar, corn meal agar, Czapek medium, and malt extract agar, and incubated at 25–30 °C for 7–10 days. Species identification was based on detailed assessment of colony morphology and microscopic features (conidiophore structure, vesicle shape, phialide arrangement, and conidial ornamentation), following CLSI guidelines. Results: Out of 709 specimens, Aspergillus species were isolated from 239 samples (33.7%). Thirteen species were identified, with A. flavus being the most common (33.4%), followed by A. niger (26.7%), A. fumigatus (19.2%), A. nidulans (4.6%), A. glaucus (3.3%), A. terreus (2.9%), A. versicolor (2.1%), A. calidoustus (1.7%), A. glabrata (1.7%), A. parasiticus (1.2%), A. clavatus (1.3%), A. ochraceus (0.8%), and A. tanneri (0.8%). A. flavus predominated among both clinical and environmental isolates. Conclusion: Conventional morphological methods combined with the use of multiple culture media proved effective for species-level identification of Aspergillus. Routine surveillance of Aspergillus species in both clinical and environmental samples can guide targeted antifungal therapy and support proactive infection control in healthcare environments.
Keywords: Aspergillus, Speciation, Differential media, Clinical specimens, Environmental surveillance
2024. Aspergillosis. Atlanta: Centers for Disease Control and Prevention (CDC) https://www.cdc.gov/aspergillosis/about/
Samson, R A, Visagie, C M, Houbraken, J, Hong, S B, Hubka, V & Klaassen, C H . 2014. Phylogeny, identification and nomenclature of the genus Aspergillus. Studies in Mycology 78(1):141–173.
Balajee, S A, Houbraken, J, Verweij, P E, Hong, S B, Yaghuchi, T, Varga, J & Samson, R A . 2007. Aspergillus species identification in the clinical setting. Studies in Mycology 59:39–46.
Hedayati, M T, Mayahi, S & Denning, D W . 2010. A study on Aspergillus species in houses of asthmatic patients from Sari city, Iran and a brief review of the health effects of exposure to indoor Aspergillus. Environmental Monitoring and Assessment 168(1-4):481–487.
Latgé, J & Chamilos, G . 2019. Aspergillus fumigatus and Aspergillosis in 2019. Clinical Microbiology Reviews 33(1):1–75.
Rudramurthy, S M, Paul, R A, Chakrabarti, A, Mouton, J W & Meis, J F . 2019. Invasive Aspergillosis by Aspergillus flavus: Epidemiology, Diagnosis, Antifungal Resistance, and Management. Journal of Fungi 5(3):1–23.
Hedayati, M T, Pasqualotto, A C, Warn, P A, Bowyer, P & Denning, D W . 2007. Aspergillus flavus: human pathogen, allergen and mycotoxin producer. Microbiology 153(6):1677–1692.
Lass-Flörl, C, Dietl, A, Kontoyiannis, D P & Brock, M . 2021. Aspergillus terreus Species Complex. Clinical Microbiology Reviews 34(4):1–27.
2021. CLSI guideline M54. Principles and Procedures for Detection and Culture of Fungi in Clinical Specimens. 2nd Edition. Clinical and Laboratory Standards Institute 1–13.
Arvanitis, M, Ziakas, P D, Zacharioudakis, I M, Zervou, F N, Caliendo, A M & Mylonakis, E . 2014. PCR in diagnosis of invasive aspergillosis: a meta-analysis of diagnostic performance. Journal of Clinical Microbiology 52(10):3731–3742.
Calderaro, A & Chezzi, C . 2024. MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory. Microorganisms 12(2):1–26.
Wickes, B L & Wiederhold, N P . 2018. Molecular diagnostics in medical mycology. Nature Communications 9(1):1–13.
Iwen, P C, Davis, J C, Reed, E C, Winfield, B A & Hinrichs, S H . 1994. Airborne fungal spore monitoring in a protective environment during hospital construction, and correlation with an outbreak of invasive aspergillosis. Infection Control & Hospital Epidemiology 15(5):303–306.
Spagnolo, A M . 2025. Aspergillus Contamination in Healthcare Facilities: An Ever-Present Issue-Prevention and Control Measures. Hygiene 5(1):1–13.
Bansal, H, Oberoi, L, Pandhi, N, Malhotra, A & Oberoi, T . 2024. Species Identification and In Vitro Antifungal Susceptibility Testing of Aspergillus Isolated from Various Clinical Samples. Asian Journal of Pharmaceutical and Clinical Research 17(10):90–92.
Chowdhary, A, Sharma, C, Kathuria, S, Hagen, F & Meis, J F . 2015. Prevalence and mechanism of triazole resistance in Aspergillus fumigatus in a referral chest hospital in Delhi, India and an update of the situation in Asia. Frontiers in Microbiology 6:1–10.
Diba, K, Kordbacheh, P, Mirhendi, S H, Rezaie, S & Mahmoudi, M . 2007. Identification of Aspergillus species using morphological characteristics. Pakistan Journal of Medical Sciences 23(6):867–872.
Valentina, Y & Umadevi, S . 2019. Phenotypic Detection and Quality Assessment of Indoor Air-Borne Microorganisms Using Passive Air Sampling Technique (Settle Plate) at A Tertiary Care Teaching Hospital in Puducherry. Journal of Pure and Applied Microbiology 13(1):241–245.
Mousavi, B, Hedayati, M T, Hedayati, N, Ilkit, M & Syedmousavi, S . 2016. Aspergillus species in indoor environments and their possible occupational and public health hazards. Current Medical Mycology 2(1):36–42.
©2025 (Shaik Meherunnisa Begum) et al. This is an open-access journal, and arƟcles are distributed under the terms of the Creative Commons Attribution License CC-BY 4.0. (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
Begum SM, Ansari MU, Arafath K M MY, Sultana J. Identification and Speciation of Aspergillus in Clinical and Environmental Samples using Culture-Based Methods: A Laboratory-based Study in Hyderabad. Perspectives in Medical Research. ;13(1):29- 34 DOI: 10.47799/pimr.1301.07