Volume: 6 Issue: 2
Year: 2018, Page: 16-20,
Introduction: The quest for the safe herbal remedy for the management of erectile dysfunction and their relationship with male sexual hormones prompted this research.
Aims and Objectives: It is aimed at determining the effect of orally administered ethanol leaf extract of P. thonningii on some reproductive hormones of male Wistar rats.
Materials and Methods :Ethanol extract of the leaf were first screened for the presence of bioactive phyto-constituent using standard methods. Twenty (20)Wistar rats were randomly assigned on the basis of average body weight into four (4) groups of five rats each following acclimatization to laboratory and handling conditions. Animals in group A (control) were administered placebo (1ml) of olive oil. Group B, C and D were administered graded doses of 50, 100 and 200mg/kg body weight of the extract respectively. Extract administration lasted for twenty one (21) days. Water and standard feeds were allowed ad libitum. At the end of dose administration, animals were sacrificed and blood obtained by cardiac puncture for reproductive hormonal profile using ELISA method and testes were removed for histological examination.
Results :The bioactive phyto-constituents identified were tannins, saponins, glycosides, flavonoids, alkaloids, carbohydrate, anthraquinones, cardiac glycosides, steroids and triterpene. The extract also produced dosage dependent increase (P<0.05) in follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone respectively following the administration of the extract with no evidence of pathological lesion on the testes.
Conclusion : It appears that the alteration in fertility hormones in the serum of treated animals might be due to the presence of bioactive constituents. It is speculated that this extract might improve sexual health, sexual activities and libido.
Keywords: Erectile dysfunction, Piliostigma thonningii, Reproductive hormones, Wistar rats
1. Badami S, Desai V B, Suresh B. Drugs play a major role in male fertility. Express Pharma Pulse 2000; 2: 18-25.
2. Ariba A.J,Oladopo O.T,Iyaniwura C.A,Dada O.A. Management of erectile dysfunction:perception and practices of Nigerian primary care clinicians .S.A Fam.Pract.2007; 49(9):16-29.
3. Nooshin Noktedan, Mehrdad Modaresi, Akbar Karimi and Aliasghar Pilevarian. The effects of hydro alcoholic extract of Boswelliacarteri on reproductive hormones in male mice. Journal of Chemical and Pharmaceutical Research 2016, 8(2):701-704.
4. Kulkarni S K, Reddy D.S. Pharmacotherapy of Male Erectile Dysfunction with Sildenafil. Ind.J.Pharmacol 1998; 30: 367-378.
5. Dasofunjo K;AsukA.A;EzugwuH.C;NwodoO.F.C and Olatunji T.L. The Aphrodisiac Effect of Piliostigma thonningii leaf on male wistar albino rats.Journal of Applied Pharmaceutical Science 2013,3(10):130-135.
6. Balamurugan K., SakthideviG.and.Mohan V.R .Stimulatory effect of the ethanol extract of Melastomamalabathricum L. (Melastomataceae) leaf on the reproductive system of male albino rats. Journal of Applied Pharmaceutical Science 2013; 3 (02): 160-165.
7. Akomolafe, S.F., Ganiyu, O.A., Akindahunsi, A.J and Akinyemi, O.A. Inhibitory Effect of Aqueous Extract of Moringaoleifera and Newbuoldialaevis Leaves on Ferrous Sulphate and Sodium Nitroprusside Induced Oxidative Stress in Rat’s Testes in Vitro. Open Journal of Medicinal Chemistry 2012; 2: 119-128.
8. Egba, Simeon I; Sunday, Godwin I. and Anaduaka, EmekaG.The effect of oral administration of aqueous extract of Newbouldialaevis leaves on fertility hormones of male albino rats .IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) 2014; 9(3): 61-64.
9. Dasofunjo K, Ipav SS and Ezugwu C.H.Antimicrobial and Bioactive Potential of Piliostigma thonningii leaf. Journal of Pure and Applied Sciences 2014; 5 (1):135-141 .
10. Harborne, J.B.Phytochemical methods, a guide to modern techniques of plant analysis. 4th ed1984, Pp.1 – 5
11. Trease GE, Evans WC. A textbook of Pharmacognosy, 13th Edition, BailliereTindall Ltd. London, 1989,pp. 19-21.
12. Dada, A.A. and Ajilore, V.O.. Use of ethanol extracts of Garcinia kola as fertility enhancer in female catfish Clariasgariepinusbroodstock. Int. J. Fish. andAquacul., 2009;1 (1): 005-010.
13. Browning, R., Schrick, F.N., Thompson, F.W. and Wakefield, T. Reproductive hormonal responses to ergotamine and ergonovine in cows during the luteal phase of the estrous cycle. Journal of Animal Sciences 1998: 76: 1448-1454.
14. Edeoga, H.O., Okwu, D.E. and Mbaebie, B.O. Phytochemical constituents of some Nigeria medicinal plants. Afr J Biotechnol 2005; 4: 685-688.
15. Yakubu, M.T., Akanji, M.A. and Oladiji, A.T. Aphrodisiac potentials of the aqueous extract of Fadogiaagrestis (Schweinf.ExHiern) stem in male albino rats. Asian J Androl 2005; 7: 399-404.
16. Bianco, F., Basini, G. and Grasselli, F. The plant alkaloid Sanguinarine affects swine granulose cell activity. Reproductive Toxicology 2006; 21: 335-340.
17. Bratoeff E, Cabeza M, Ramirez E, Heuze Y, Flores E, : Recent advances in the chemistry and pharmacological activity of new steroidal antiandrogens and 5 alpha-reductase inhibitors. Current Medicinal Chemistry 2005; 12 (8): 927– 43.
18. Amanatkar H.R, Chibnall J.T, Seo B.W,Manepalli J.N, Grossberg G.T. Impact of exogenous testosterone on mood: a systematic review and meta-analysis of randomized placebo-controlled trials. Annals of Clinical Psychiatry 2014; 26 (1): 19–32.
19. MansourehEmadi,MehrdadModaresi, Akbar Karimi and AliasgharPilevaria De .The effects of frankincense’s hydro alcoholic extract on pituitary-gonadal axis in female mice Journal of Chemical and Pharmaceutical Research 2016;8(2):697-700.
20. Harding S.M, Velotta J.P . Comparing the relative amount of testosterone required to restore sexual arousal, motivation, and performance in male rats. Hormones and Behavior 2011; 59 (5): 666–73.
21. Colao, A., Vitale, G., Di Sarno, A., Spiezia, S., Guerra, E., Ciccarelli, A. and Lombardi, G. Prolactin and prostate hypertrophy: a pilot observational, prospective, casecontrol study in men with prolactinoma. Journal of Clinical Endocrinology and Metabolism 2004; 89: 2770–2775.
22. Nguyen T.V, McCracken J.T, Albaugh M.D, Botteron K.N, Hudziak J.J, Ducharme S. A testosterone-related structural brain phenotype predicts aggressive behavior from childhood to adulthood. Psycho neuroendocrinology 2016;63: 109–18.
Dasofunjo. K, Nwodo O F C , Ezugwu H.C, Atunka E.A, Onah L.N. Impact of oral administration of Ethanol leaf extract of Piliostigma thonningii on fertility hormones of male Wistar rats. Perspectives in Medical Research 2018;6(2):16-20.