Volume: 10 Issue: 1
Year: 2022, Page: 31-34, Doi: https://doi.org/10.47799/pimr.1001.05
Received: Nov. 4, 2021 Accepted: Feb. 7, 2022 Published: Feb. 7, 2022
Background: In India, malaria persist throughout the year and usually the incidence is more during rainy season. This necessitates the use of other laboratory methods to clinch the diagnosis, which is important in view of frequent relapses reported in vivax malaria and hence there is need for radical treatment. Considering all the above factors, a need is felt to evaluate the available lab diagnostic procedures for malaria, and also to know the trend of malaria epidemic at Warangal.
Methods: It was a prospective study, conducted in the department of Microbiology, KMC Warangal. Paediatric age group with fever with chills and rigors followed by sweating were included in this research. Blood sample was collected thick and thin blood smears were prepared, stained by JSB or Leishmans or Giemsa. Parasight 'F' test and OptiMAL tests were used to detect the antigen. Chi square test was used to find the correlation between the parameters.
Results: Total 300 blood samples were collected, malaria positivity was 24%, male female ratio was 1.05, statistically there was no significant difference. In area wise malaria positivity also statistically there was no significant difference. Age wise, maximum cases were diagnosed in 6 – 10 years group. Parasight F test was identified to be highly sensitive in the diagnosis of malaria among the paediatric age group.
Conclusion: Falciparum malaria is identified to be the commonest malaria in this area which effect all the paediatric age groups and gender. Parasight F test was found to be the better technique in malaria diagnosis.
Keywords: Malaria, plasmodium, age, study
Murray, Cjl & Lopez, A D . 1996. Evidence-based health policy lessons from the Global Burden of Disease Study. Science 274:740–743.
Murray, Cjl & Lopez, A D . 1997. The Global Burden of Disease 1990-2020: alternative projections of mortality and disability by cause for eight regions. Lancet 349:1498–1504.
Kumar, Ashwani, Neenavalecha, Tanu, Jain, Aditya P & Dash, . 2007. Burden of Malaria in India: Retrospective and Prospective View. Am J Trop Med Hyg 77(6):69–78.
Kiran, K, Dayanand, Kishore, Punnath, , Valleeshachandrashekar, Rajeshwara N, Achur, Srinivas B, Kakkilaya, , Susanta, K, Ghosh, , Suchethakumari, D & Channegowda, . 2017. Malaria prevalence in Mangaluru city area in the southwestern coastal region of India. Malaria J 16:492–501.
Monica, Cheesbrough . 2006. District Laboratory Practical in Tropical Countries. Edinburg building, Trumpington street, Cambridge CB2 1IR, United Kingdom: Cambridge University Press
JM, Crutcher . 1996. Malaria. In: S, Baron , ed. Medical Microbiology (4). Texas: University of Texas Medical Branch at Galveston
Rapid Test for P. falciparum malaria. Tulip Diagnostics (P) Ltd. http://www.tulipgroup.com/Orchid_New/html/product_specs/para_device.htm
Chayani, N, Das, B, Sur, M S & Bajoria, . 2004. Comparison of parasite lactate dehydrogenase based immunochromatografic antigen detection assay (OptiMal®) with microscopy for detection of malaria parasites. Ind J Med Microb 22(2):104–110.
Ritabratakundu, , Nupurganguly, Tapan, Kr, , Ghosh, Panna, Choudhury, , Raju, C & Shah, . 2005. Diagnosis and management of malaria in children. Indian Pediatrics 42:1101–1115.
Tripathy, R, Parida, S, Das, L, Mishra, D P, Tripathy, D, Das, M C, Chen, H, Maguire, J H & Panigrahi, P . 2007. Clinical manifestations and predictors of severe malaria in Indian children. Pediatrics 120(3):454–60.
Squire, D S, Asmah, R H, Brown, C A, Adjei, D N, Obeng-Nkrumah, N & Pf, Ayeh-Kumi . 2016. Effect of Plasmodium falciparum malaria parasites on haematological parameters in Ghanaian children. J Parasit Dis 40(2):303–314.
Tyagi, P, Roy, A & Malhotra, M S . 2005. Knowledge, awareness and practices towards malaria in communities of rural, semi-rural and bordering areas of east Delhi (India) J Vector Borne Dis 42:30–35.
Maria Van Eijk, Anna, Sutton, Patrick L, Steven, Lalitharamanathapuram, Sullivan, A & Kanagaraj, Deena . 2019. The burden of submicroscopic and asymptomatic malaria in India revealed from epidemiology studies at three varied transmission sites in India. Scientific Rep 9:17095.
Dk Mendiratta, , Kbhutada, & Narang, . 2006. Evaluation of different methods for diagnosis of P.Falciparum malaria. Ind J Med Microbiol 24(1):49–51.
Mills, C D, Burgess, D C, Taylor, H J & Kain, K C . 1999. Evaluation of rapid and (4) inexpensive dipstick assay for the diagnosis of P. falciparum malaria. Bull World Health Org 77(7).
N, Dasarwar, vatchala, Sudha, Dasari, Swamy Naidu, pothula, Somasekhar & Jayachandra, . 2021. A study to find the commonest plasmodium malaria in Srikakulam district. Andhra Pradesh. Inter J Health &Clin Rese 4(17):210–212.
Amgothu M, Akkala V, Bandi S, T JC, Vatchala S. Incidence of Malaria among the Pediatric Age Group Attending to MGM Hospital Warangal. Perspectives in Medical Research. 2022;10(1):31-34 DOI: 10.47799/pimr.1001.05