Volume: 12 Issue: 2
Year: 2024, Page: 36-41, Doi: https://doi.org/10.47799/pimr.1202.07
Received: March 14, 2024 Accepted: April 14, 2024 Published: Aug. 20, 2024
Introduction: Various types of metaplastic changes, such as gastric metaplasia (foveolar type or antral type) and intestinal metaplasia, are observed in chronic cholecystitis but not in normal mucosa. Helicobacter species can be detected in the bile and gallbladder tissue of patients with benign gallbladder disease. Objective: The present study aimed to observe the correlation between chronic cholecystitis and the presence of Helicobacter pylori as well as the different types of metaplastic changes in gallbladder mucosa. Materials and Methods: This descriptive, observational study was conducted at the Departments of Pathology and Surgery at a tertiary care medical college and hospital from April 2021 to March 2022, using a cross-sectional design. All confirmed cases of chronic cholecystitis, with or without cholelithiasis, were included in the study. Results: Intestinal metaplasia was the most common type of metaplastic change in the gallbladder epithelium in chronic cholecystitis in this region. PAS and Alcian Blue positivity were significantly higher in cases with metaplasia compared to those without metaplasia. H. pylori was detected in 3 cases (1.55%) of chronic cholecystitis in this study population. Conclusion: Mucin histochemistry may help identify early metaplastic changes in gallbladder epithelium. The use of a combination of methods for the detection of H. pylori in gallbladder tissue may increase the detection of positive cases.
Keywords: Helicobacter pylori, PAS, Alcian Blue, chronic cholecystitis, metaplasia
Latio, M . 1976. Early carcinoma of the gallbladder. Beitr Pathol 158:169–72.
Latio, M . 1983. Histogenesis of epithelial neoplasms of the human gallbladder, I Dysplasia. Pathol Res Pract 178:51–57.
Dowling, G P & Kelly, J K . 1986. The histogenesis of adenocarcinoma of the gallbladder. Cancer 58:1702–1710.
Weedon, D . 1994. Diseases of the gallbladder. In: McSween RMN, Antony PP, Bua AD & et al , eds. Pathology of the Liver. (pp. 514-534)
Bilhartz, L E & Horton, J D . 1998. Feldman M, Scharschmidt BF & Sleisenger MH , eds. Sleisenger and Fordtran's Gastrointestinal and Liver Disease: Pathophysiology/Diagnosis/Management. 6th ed. Philadelphia: WB Saunders. (pp. 948-972)
Jagannath, S B, Singh, V K & Cruz-Correa, M . 2003. A long-term cohort study of outcome after cholecystectomy for chronic acalculous cholecystitis. Am J Surg 185:91–95.
Manoj, P . 2007. Helicobacter species are associated with a possible increase in risk of biliary lithiasis and benign biliary diseases. World J Surg Oncol 5:94.
Myung, S J, Kim, M H, Shim, K N, Kim, Y S, Kim, E O & Kim, H J . 2000. Detection of Helicobacter pylori DNA in the human biliary tree and its association with hepatolithiasis. Dig Dis Sci 45:1405–141.
Mohan, H, Punia, Rps, Dhawan, S B, Ahai, S & Sekhon, M S . 2005. Morphological spectrum of gallstone disease in 1100 cholecystectomies in North India. Indian J Surg 67:140–142.
Tandon, R K . 1988. Pathogenesis of gallstones in India. Trop Gastroenterol 9:83–91.
Baig, S J, Biswas, S, Das, S, Basu, K & Chattopadhyay, G . 2002. Histopathological changes in gallbladder mucosa in cholelithiasis: correlation with chemical composition of gallstones. Trop Gastroenterol 23:25–32.
Dhar, S C, Ansari, S, Saha, M, Ahmad, M M, Rahman, M T & Hasan, M . 2001. Gallstone disease in a rural Bangladesh community. Indian J Gastroenterol 20:223–229.
Raptopoulos, V, Compton, C C & Doherty, P . 1986. Chronic acalculous gallbladder disease: Multiimaging evaluation with clinical-pathologic correlation. AJR Am J Roentgenol 147:721–724.
Barcia, J . 2003. Histologic analysis of chronic inflammatory patterns in the gallbladder: diagnostic criteria for reporting cholecystitis. Ann Diagn Pathol 7(3):147–153.
Albores-Saavedra, J, Vazquez, Alcántara-, Cruz-Ortiz, A, Herrera-Goepfert, H & R . 1980. The precursor lesions of invasive gallbladder carcinoma. Hyperplasia, atypical hyperplasia, and carcinoma in situ. Cancer 45(5):919–927.
Yamaguchi, K & Enjoji, M . 1988. Carcinoma of the gallbladder: A clinicopathology of 103 patients and a newly proposed staging. Cancer 62(7):1425–1432.
Martinez-Guzman, E & De La Rosa-Bayan, J . 1998. Neoplasms and dysplasia of the gallbladder and their relationship with lithiasis: A case-control clinicopathological study. Rev Gastroenterol Mex 63:82–90.
Duarte, I, Llanos, O, Domke, H, Harz, C & Valdivieso, V . 1993. Metaplasia and precursor lesions of gallbladder carcinoma: Frequency, distribution, and probability of detection in routine histologic samples. Cancer 72(6):1878–1884.
Yamagiwa, H & Tomiyama, H . 1987. Intestinal metaplasia, dysplasia, carcinoma sequence of the gallbladder. Acta Pathol Jpn 36:989–97.
Stancu, M, Căruntu, I D, Giuşcă, S & Dobrescu, G . 2007. Hyperplasia, metaplasia, dysplasia and neoplasia lesions in chronic cholecystitis - a morphologic study. Rom J Morphol Embryol 48(4):335–342.
Khanna, R, Chansuria, R, Kumar, M & Shukla, H S . 2006. Histological changes in the gallbladder due to stone disease. Indian J Surg 68:201–205.
Gupta, S C, Misra, V, Singh, P A, Roy, A, Misra, S P & Gupta, A K . 2000. Gallstones and carcinoma gallbladder. Indian J Pathol Microbiol 43(1):147–154.
Lin, T T, Yeh, C T, Wu, C S & Liaw, Y F . 1995. Detection and partial sequence analysis of Helicobacter pylori DNA in bile samples. Dig Dis Sci 40:2214–2223.
Fox, J G, Dewhirst, F E, Shen, Z, Feng, Y, Taylor, N S & Paster, B J . 1998. Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology 114:755–763.
Bansal, V K, Misra, M C, Chaubal, G, Gupta, Datta, Das, S, Ahuja, B, Sagar, V & S . 2012. Helicobacter pylori in gallbladder mucosa in patients with gallbladder disease. Indian J Gastroenterol 31(2):57–60.
Yakoob, J, Khan, M R, Abbas, Z, Jafri, W, Azmi, R & Ahmad, Z . 2011. Helicobacter pylori: association with gallbladder disorders in Pakistan. Br J Biomed Sci 68(2):59–64.
Misra, V, Misra, S P, Dwivedi, M, Shouche, Y, Dharne, M & Singh, P A . 2007. Helicobacter pylori in areas of gastric metaplasia in the gallbladder and isolation of H. pylori DNA from gallstones. Pathology 39(4):419–443.
©2024 (Rabiul Alam) et al. This is an open-access journal, and articles are distributed under the terms of the Creative Commons Attribution License CC-BY 4.0. (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
Alam R, Sarkar SK, Bandyopadhyay S, ChakrabarƟ S, Ghosh S. Helicobacter Pylori and Metaplastic Changes in Chronic Cholecytitis: A Correlation Study. Perspectives in Medical Research. 2024;12(2):36-41 DOI: 10.47799/pimr.1202.07